AKT3 Antibody (PACO54086)
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주문- SKU:
- PACO54086
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | AKT3 Antibody (PACO54086) |
Antibody SKU: | PACO54086 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IP |
Recommended Dilutions: | ELISA:1:2000-1:10000, IP:1:200-1:2000 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human RAC-γ serine/threonine-protein kinase protein (1-164AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunoprecipitating AKT3 in mouse brain whole cell lysate. Lane 1: Rabbit control IgG (1µg) instead of PACO54086 in mouse brain whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO54086 (6µg) + Mouse brain whole cell lysate (500µg). Lane 3: Mouse brain whole cell lysate (10µg). |
Background: | AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. |
Synonyms: | RAC-γ serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase Akt-3) (Protein kinase B gamma) (PKB gamma) (RAC-PK-gamma) (STK-2), AKT3, PKBG |
UniProt Protein Function: | Akt3: an AGC kinase that plays a critical role in controlling the balance between survival and apoptosis. Stimulated by platelet-derived growth factor, insulin, and insulin-like growth factor 1. Alternatively spliced isoforms have been described. |
UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.1; AGC group; AKT family Chromosomal Location of Human Ortholog: 1q44 Cellular Component: cytoplasm; Golgi apparatus; intracellular; nucleoplasm; plasma membrane Molecular Function:ATP binding; protein binding; protein kinase activity; protein serine/threonine kinase activity Biological Process: mitochondrial genome maintenance; peptidyl-serine phosphorylation; protein amino acid phosphorylation; signal transduction Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 2 |
NCBI Summary: | The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q9Y243 |
NCBI GenInfo Identifier: | 12643943 |
NCBI Gene ID: | 10000 |
NCBI Accession: | Q9Y243.1 |
UniProt Secondary Accession: | Q9Y243,Q0VAA6, Q5VTI1, Q5VTI2, Q96QV3, Q9UFP5, |
UniProt Related Accession: | Q9Y243 |
Molecular Weight: | 54,032 Da |
NCBI Full Name: | RAC-gamma serine/threonine-protein kinase |
NCBI Synonym Full Names: | AKT serine/threonine kinase 3 |
NCBI Official Symbol: | AKT3 |
NCBI Official Synonym Symbols: | MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma |
NCBI Protein Information: | RAC-gamma serine/threonine-protein kinase |
UniProt Protein Name: | RAC-gamma serine/threonine-protein kinase |
UniProt Synonym Protein Names: | Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2 |
Protein Family: | RAC-gamma serine/threonine-protein kinase |
UniProt Gene Name: | AKT3 |
UniProt Entry Name: | AKT3_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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