ADGRG1 Antibody (PACO59225)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO59225
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | ADGRG1 Antibody (PACO59225) |
Antibody SKU: | PACO59225 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Adhesion G-protein coupled receptor G1 protein (26-216AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO59225 diluted at 1:400 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of MCF-7 cells with PACO59225 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO59225 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Receptor involved in cell adhesion and probably in cell-cell interactions. Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. Receptor for collagen III/COL3A1 in the developing brain and involved in regulation of cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination (By similarity). Binding to the COL3A1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to GNA13 and possibly GNA12. Plays a role in the maintenance of hematopoietic stem cells and/or leukemia stem cells in bone marrow niche (By similarity). Plays a critical role in cancer progression by inhibiting VEGFA production threreby inhibiting angiogenesis through a signaling pathway mediated by PRKCA. Plays an essential role in testis development (By similarity). |
Synonyms: | Adhesion G-protein coupled receptor G1 (G-protein coupled receptor 56) (Protein TM7XN1) [Cleaved into: ADGRG1 N-terminal fragment (ADGRG1 NT) (GPR56 N-terminal fragment) (GPR56 NT) (GPR56(N)) (GPR56 extracellular subunit) (GPR56 subunit alpha); ADGRG1 C-terminal fragment (ADGRG1 CT) (GPR56 C-terminal fragment) (GPR56 CT) (GPR56(C)) (GPR56 seven-transmembrane subunit) (GPR56 7TM) (GPR56 subunit beta)], ADGRG1, GPR56 TM7LN4 TM7XN1 |
UniProt Protein Function: | GPR56: Could be involved in cell-cell interactions. Defects in GPR56 are the cause of bilateral frontoparietal polymicrogyria (BFPP). BFPP is characterized by disorganized cortical lamination that is most severe in frontal cortex. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:GPCR, family 2; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; Cell adhesion Chromosomal Location of Human Ortholog: 16q21 Cellular Component: integral to membrane; integral to plasma membrane; intracellular Molecular Function:collagen binding; extracellular matrix binding; G-protein coupled receptor activity Biological Process: angiogenesis; brain development; cell adhesion; cell surface receptor linked signal transduction; cell-cell signaling; cerebral cortex radial glia guided migration; cerebral cortex regionalization; G-protein coupled receptor protein signaling pathway; layer formation in the cerebral cortex; negative regulation of cell proliferation; positive regulation of cell adhesion; positive regulation of Rho protein signal transduction; Rho protein signal transduction Disease: Polymicrogyria, Bilateral Frontoparietal; Polymicrogyria, Bilateral Perisylvian, Autosomal Recessive |
NCBI Summary: | This gene encodes a member of the G protein-coupled receptor family and regulates brain cortical patterning. The encoded protein binds specifically to transglutaminase 2, a component of tissue and tumor stroma implicated as an inhibitor of tumor progression. Mutations in this gene are associated with a brain malformation known as bilateral frontoparietal polymicrogyria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
UniProt Code: | Q9Y653 |
NCBI GenInfo Identifier: | 45476992 |
NCBI Gene ID: | 9289 |
NCBI Accession: | Q9Y653.2 |
UniProt Secondary Accession: | Q9Y653,O95966, Q6ZMP1, Q8NGB3, Q96HB4, A6NIT7, A6NJV9 B0M0K4, B4DR54, |
UniProt Related Accession: | Q9Y653 |
Molecular Weight: | 58,042 Da |
NCBI Full Name: | G-protein coupled receptor 56 |
NCBI Synonym Full Names: | adhesion G protein-coupled receptor G1 |
NCBI Official Symbol: | ADGRG1 |
NCBI Official Synonym Symbols: | BFPP; BPPR; GPR56; TM7LN4; TM7XN1 |
NCBI Protein Information: | G-protein coupled receptor 56 |
UniProt Protein Name: | Adhesion G-protein coupled receptor G1 |
UniProt Synonym Protein Names: | G-protein coupled receptor 56; Protein TM7XN1 |
UniProt Gene Name: | ADGRG1 |
UniProt Entry Name: | AGRG1_HUMAN |